Construct: shRNA TRCN0000413741

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033046.2-1789s21c1
DNA Barcode:
TTTCTTTGACATGAGCCAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RTKN (6242)
Gene Description:
rhotekin
Transcript:
RefSeq NM_033046.2 (CURRENT)
Match location:
Position 1789 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000413741)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTCTTTGACATGAGCCAATG
Hairpin Sequence:
5'-CCGG-TTTCTTTGACATGAGCCAATG-CTCGAG-CATTGGCTCATGTCAAAGAAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTCTTTGACATGAGCCAATGCTCGAGCATTGGCTCATGTCAAAGAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTCTTTGACATGAGCCAATGCTCGAGCATTGGCTCATGTCAAAGAAA-3'

Other clones with same target sequence:

(none)