Construct: shRNA TRCN0000413747

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003575.2-1148s21c1
DNA Barcode:
ATATTCCCACGGATCCCAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF282 (8427)
Gene Description:
zinc finger protein 282
Transcript:
RefSeq NM_003575.2 (NON-CURRENT)
Match location:
Position 1148 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000413747)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATATTCCCACGGATCCCAATT
Hairpin Sequence:
5'-CCGG-ATATTCCCACGGATCCCAATT-CTCGAG-AATTGGGATCCGTGGGAATAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATATTCCCACGGATCCCAATTCTCGAGAATTGGGATCCGTGGGAATATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATATTCCCACGGATCCCAATTCTCGAGAATTGGGATCCGTGGGAATAT-3'

Other clones with same target sequence:

(none)