Construct: shRNA TRCN0000413788

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144711.4-503s21c1
DNA Barcode:
AGCTGCTTGCAGCAATTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KLHL23 (151230)
Gene Description:
kelch like family member 23
Transcript:
RefSeq NM_144711.4 (NON-CURRENT)
Match location:
Position 503 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000413788)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCTGCTTGCAGCAATTATTT
Hairpin Sequence:
5'-CCGG-AGCTGCTTGCAGCAATTATTT-CTCGAG-AAATAATTGCTGCAAGCAGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCTGCTTGCAGCAATTATTTCTCGAGAAATAATTGCTGCAAGCAGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCTGCTTGCAGCAATTATTTCTCGAGAAATAATTGCTGCAAGCAGCT-3'

Other clones with same target sequence:

(none)