Construct: shRNA TRCN0000413792

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_052956.2-1174s21c1
DNA Barcode:
AGTCGGAAACGGGACTAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACSM1 (116285)
Gene Description:
acyl-CoA synthetase medium chain family member 1
Transcript:
RefSeq NM_052956.2 (NON-CURRENT)
Match location:
Position 1174 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000413792)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTCGGAAACGGGACTAATTT
Hairpin Sequence:
5'-CCGG-AGTCGGAAACGGGACTAATTT-CTCGAG-AAATTAGTCCCGTTTCCGACT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTCGGAAACGGGACTAATTTCTCGAGAAATTAGTCCCGTTTCCGACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTCGGAAACGGGACTAATTTCTCGAGAAATTAGTCCCGTTTCCGACT-3'

Other clones with same target sequence:

(none)