Construct: shRNA TRCN0000413802

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020383.3-358s21c1
DNA Barcode:
GCTCATCAGAGTGAGTATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
XPNPEP1 (7511)
Gene Description:
X-prolyl aminopeptidase 1
Transcript:
RefSeq NM_020383.3 (NON-CURRENT)
Match location:
Position 358 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000413802)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCATCAGAGTGAGTATATT
Hairpin Sequence:
5'-CCGG-GCTCATCAGAGTGAGTATATT-CTCGAG-AATATACTCACTCTGATGAGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCATCAGAGTGAGTATATTCTCGAGAATATACTCACTCTGATGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCATCAGAGTGAGTATATTCTCGAGAATATACTCACTCTGATGAGC-3'

Other clones with same target sequence:

(none)