Construct: shRNA TRCN0000413809

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014653.2-2866s21c1
DNA Barcode:
GTCCCTTCTGGGCTGAGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WSCD2 (9671)
Gene Description:
WSC domain containing 2
Transcript:
RefSeq NM_014653.2 (NON-CURRENT)
Match location:
Position 2866 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000413809)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCCCTTCTGGGCTGAGATTT
Hairpin Sequence:
5'-CCGG-GTCCCTTCTGGGCTGAGATTT-CTCGAG-AAATCTCAGCCCAGAAGGGAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCCCTTCTGGGCTGAGATTTCTCGAGAAATCTCAGCCCAGAAGGGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCCCTTCTGGGCTGAGATTTCTCGAGAAATCTCAGCCCAGAAGGGAC-3'

Other clones with same target sequence:

(none)