Construct: shRNA TRCN0000413833

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019106.5-701s21c1
DNA Barcode:
TGGATCTTGAGTTCATGAAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SEPTIN3 (55964)
Gene Description:
septin 3
Transcript:
RefSeq NM_019106.5 (CURRENT)
Match location:
Position 701 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000413833)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGATCTTGAGTTCATGAAAC
Hairpin Sequence:
5'-CCGG-TGGATCTTGAGTTCATGAAAC-CTCGAG-GTTTCATGAACTCAAGATCCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGATCTTGAGTTCATGAAACCTCGAGGTTTCATGAACTCAAGATCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGATCTTGAGTTCATGAAACCTCGAGGTTTCATGAACTCAAGATCCA-3'

Other clones with same target sequence:

(none)