Construct: shRNA TRCN0000413876

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019789.3-1152s21c1
DNA Barcode:
GCCTATTCCTAGGTCAATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcnip3 (56461)
Gene Description:
Kv channel interacting protein 3, calsenilin
Transcript:
RefSeq NM_019789.3 (NON-CURRENT)
Match location:
Position 1152 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000413876)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTATTCCTAGGTCAATAAA
Hairpin Sequence:
5'-CCGG-GCCTATTCCTAGGTCAATAAA-CTCGAG-TTTATTGACCTAGGAATAGGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTATTCCTAGGTCAATAAACTCGAGTTTATTGACCTAGGAATAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTATTCCTAGGTCAATAAACTCGAGTTTATTGACCTAGGAATAGGC-3'

Other clones with same target sequence:

(none)