Construct: shRNA TRCN0000413898

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012174.1-438s21c1
DNA Barcode:
GAGCCAAGGAACACATGTTAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FBXW8 (26259)
Gene Description:
F-box and WD repeat domain containing 8
Transcript:
RefSeq NM_012174.1 (CURRENT)
Match location:
Position 438 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000413898)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCCAAGGAACACATGTTAC
Hairpin Sequence:
5'-CCGG-GAGCCAAGGAACACATGTTAC-CTCGAG-GTAACATGTGTTCCTTGGCTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCCAAGGAACACATGTTACCTCGAGGTAACATGTGTTCCTTGGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCCAAGGAACACATGTTACCTCGAGGTAACATGTGTTCCTTGGCTC-3'

Other clones with same target sequence:

(none)