Construct: shRNA TRCN0000414063

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144643.2-2453s21c1
DNA Barcode:
CAGACTTCAAAGGCGTCTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCLT1 (132320)
Gene Description:
sodium channel and clathrin linker 1
Transcript:
RefSeq NM_144643.2 (NON-CURRENT)
Match location:
Position 2453 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000414063)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGACTTCAAAGGCGTCTAAG
Hairpin Sequence:
5'-CCGG-CAGACTTCAAAGGCGTCTAAG-CTCGAG-CTTAGACGCCTTTGAAGTCTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGACTTCAAAGGCGTCTAAGCTCGAGCTTAGACGCCTTTGAAGTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGACTTCAAAGGCGTCTAAGCTCGAGCTTAGACGCCTTTGAAGTCTG-3'

Other clones with same target sequence:

(none)