Construct: shRNA TRCN0000414126

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009051.3-526s21c1
DNA Barcode:
ACCGAAAGAAGTCTTCTAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rex2 (19715)
Gene Description:
reduced expression 2
Transcript:
RefSeq NM_009051.3 (NON-CURRENT)
Match location:
Position 526 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000414126)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCGAAAGAAGTCTTCTAAAT
Hairpin Sequence:
5'-CCGG-ACCGAAAGAAGTCTTCTAAAT-CTCGAG-ATTTAGAAGACTTCTTTCGGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCGAAAGAAGTCTTCTAAATCTCGAGATTTAGAAGACTTCTTTCGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCGAAAGAAGTCTTCTAAATCTCGAGATTTAGAAGACTTCTTTCGGT-3'

Other clones with same target sequence:

(none)