Construct: shRNA TRCN0000414211

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172671.2-3587s21c1
DNA Barcode:
TGATGTATGGCATAGCAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lgr4 (107515)
Gene Description:
leucine-rich repeat-containing G protein-coupled receptor 4
Transcript:
RefSeq NM_172671.2 (CURRENT)
Match location:
Position 3587 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000414211)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATGTATGGCATAGCAATAT
Hairpin Sequence:
5'-CCGG-TGATGTATGGCATAGCAATAT-CTCGAG-ATATTGCTATGCCATACATCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATGTATGGCATAGCAATATCTCGAGATATTGCTATGCCATACATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATGTATGGCATAGCAATATCTCGAGATATTGCTATGCCATACATCA-3'

Other clones with same target sequence:

(none)