Construct: shRNA TRCN0000414218

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018222.3-1630s21c1
DNA Barcode:
ACTAAGGCTTGGAGGTTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PARVA (55742)
Gene Description:
parvin alpha
Transcript:
RefSeq NM_018222.3 (NON-CURRENT)
Match location:
Position 1630 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000414218)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTAAGGCTTGGAGGTTAAAT
Hairpin Sequence:
5'-CCGG-ACTAAGGCTTGGAGGTTAAAT-CTCGAG-ATTTAACCTCCAAGCCTTAGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTAAGGCTTGGAGGTTAAATCTCGAGATTTAACCTCCAAGCCTTAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTAAGGCTTGGAGGTTAAATCTCGAGATTTAACCTCCAAGCCTTAGT-3'

Other clones with same target sequence:

(none)