Construct: shRNA TRCN0000414251

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019014.4-4389s21c1
DNA Barcode:
TTATAGCCACGTTGAAGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
POLR1B (84172)
Gene Description:
RNA polymerase I subunit B
Transcript:
RefSeq NM_019014.4 (NON-CURRENT)
Match location:
Position 4389 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000414251)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTATAGCCACGTTGAAGTAAA
Hairpin Sequence:
5'-CCGG-TTATAGCCACGTTGAAGTAAA-CTCGAG-TTTACTTCAACGTGGCTATAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTATAGCCACGTTGAAGTAAACTCGAGTTTACTTCAACGTGGCTATAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTATAGCCACGTTGAAGTAAACTCGAGTTTACTTCAACGTGGCTATAA-3'

Other clones with same target sequence:

(none)