Construct: shRNA TRCN0000414271

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152594.2-1758s21c1
DNA Barcode:
GAATCTTGCCTGGTATCATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPRED1 (161742)
Gene Description:
sprouty related EVH1 domain containing 1
Transcript:
RefSeq NM_152594.2 (NON-CURRENT)
Match location:
Position 1758 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000414271)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATCTTGCCTGGTATCATTG
Hairpin Sequence:
5'-CCGG-GAATCTTGCCTGGTATCATTG-CTCGAG-CAATGATACCAGGCAAGATTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATCTTGCCTGGTATCATTGCTCGAGCAATGATACCAGGCAAGATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATCTTGCCTGGTATCATTGCTCGAGCAATGATACCAGGCAAGATTC-3'

Other clones with same target sequence:

(none)