Construct: shRNA TRCN0000414279

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198849.2-1060s21c1
DNA Barcode:
GTCTGCCATGGGCATTATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SIAH3 (283514)
Gene Description:
siah E3 ubiquitin protein ligase family member 3
Transcript:
RefSeq NM_198849.2 (NON-CURRENT)
Match location:
Position 1060 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000414279)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTGCCATGGGCATTATATT
Hairpin Sequence:
5'-CCGG-GTCTGCCATGGGCATTATATT-CTCGAG-AATATAATGCCCATGGCAGAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTGCCATGGGCATTATATTCTCGAGAATATAATGCCCATGGCAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTGCCATGGGCATTATATTCTCGAGAATATAATGCCCATGGCAGAC-3'

Other clones with same target sequence:

(none)