Construct: shRNA TRCN0000414312

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130863.1-2554s21c1
DNA Barcode:
CTCTTGCCACTGGTGAGAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Grk2 (110355)
Gene Description:
G protein-coupled receptor kinase 2
Transcript:
RefSeq NM_130863.1 (NON-CURRENT)
Match location:
Position 2554 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000414312)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTTGCCACTGGTGAGAGAA
Hairpin Sequence:
5'-CCGG-CTCTTGCCACTGGTGAGAGAA-CTCGAG-TTCTCTCACCAGTGGCAAGAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTTGCCACTGGTGAGAGAACTCGAGTTCTCTCACCAGTGGCAAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTTGCCACTGGTGAGAGAACTCGAGTTCTCTCACCAGTGGCAAGAG-3'

Other clones with same target sequence:

(none)