Construct: shRNA TRCN0000414329

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144964.2-641s21c1
DNA Barcode:
TAGTTACCTGTTAGACATAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRMT10B (158234)
Gene Description:
tRNA methyltransferase 10B
Transcript:
RefSeq NM_144964.2 (NON-CURRENT)
Match location:
Position 641 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000414329)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGTTACCTGTTAGACATAAC
Hairpin Sequence:
5'-CCGG-TAGTTACCTGTTAGACATAAC-CTCGAG-GTTATGTCTAACAGGTAACTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGTTACCTGTTAGACATAACCTCGAGGTTATGTCTAACAGGTAACTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGTTACCTGTTAGACATAACCTCGAGGTTATGTCTAACAGGTAACTA-3'

Other clones with same target sequence:

(none)