Construct: shRNA TRCN0000414378

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018233.3-518s21c1
DNA Barcode:
TGACATGTCCTGTGCTAAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OGFOD1 (55239)
Gene Description:
2-oxoglutarate and iron dependent oxygenase domain containing 1
Transcript:
RefSeq NM_018233.3 (NON-CURRENT)
Match location:
Position 518 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000414378)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGACATGTCCTGTGCTAAATA
Hairpin Sequence:
5'-CCGG-TGACATGTCCTGTGCTAAATA-CTCGAG-TATTTAGCACAGGACATGTCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGACATGTCCTGTGCTAAATACTCGAGTATTTAGCACAGGACATGTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGACATGTCCTGTGCTAAATACTCGAGTATTTAGCACAGGACATGTCA-3'

Other clones with same target sequence:

(none)