Construct: shRNA TRCN0000414411

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172545.3-1625s21c1
DNA Barcode:
GAGAGCGTGGATCACGAATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ehmt1 (77683)
Gene Description:
euchromatic histone methyltransferase 1
Transcript:
RefSeq NM_172545.3 (NON-CURRENT)
Match location:
Position 1625 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000414411)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAGCGTGGATCACGAATTG
Hairpin Sequence:
5'-CCGG-GAGAGCGTGGATCACGAATTG-CTCGAG-CAATTCGTGATCCACGCTCTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAGCGTGGATCACGAATTGCTCGAGCAATTCGTGATCCACGCTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAGCGTGGATCACGAATTGCTCGAGCAATTCGTGATCCACGCTCTC-3'

Other clones with same target sequence:

(none)