Construct: shRNA TRCN0000414420

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032226.2-580s21c1
DNA Barcode:
ATATTGAGAAGCCTAAATCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZCCHC7 (84186)
Gene Description:
zinc finger CCHC-type containing 7
Transcript:
RefSeq NM_032226.2 (CURRENT)
Match location:
Position 580 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000414420)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATATTGAGAAGCCTAAATCTG
Hairpin Sequence:
5'-CCGG-ATATTGAGAAGCCTAAATCTG-CTCGAG-CAGATTTAGGCTTCTCAATAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATATTGAGAAGCCTAAATCTGCTCGAGCAGATTTAGGCTTCTCAATATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATATTGAGAAGCCTAAATCTGCTCGAGCAGATTTAGGCTTCTCAATAT-3'

Other clones with same target sequence:

(none)