Construct: shRNA TRCN0000414490

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006633.2-2625s21c1
DNA Barcode:
ACTAGAGGTTGCACGATTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IQGAP2 (10788)
Gene Description:
IQ motif containing GTPase activating protein 2
Transcript:
RefSeq NM_006633.2 (NON-CURRENT)
Match location:
Position 2625 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000414490)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTAGAGGTTGCACGATTAAG
Hairpin Sequence:
5'-CCGG-ACTAGAGGTTGCACGATTAAG-CTCGAG-CTTAATCGTGCAACCTCTAGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTAGAGGTTGCACGATTAAGCTCGAGCTTAATCGTGCAACCTCTAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTAGAGGTTGCACGATTAAGCTCGAGCTTAATCGTGCAACCTCTAGT-3'

Other clones with same target sequence:

(none)