Construct: shRNA TRCN0000414599

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031898.2-845s21c1
DNA Barcode:
CTGACGGAAGTTGATACTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TEKT3 (64518)
Gene Description:
tektin 3
Transcript:
RefSeq NM_031898.2 (NON-CURRENT)
Match location:
Position 845 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000414599)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGACGGAAGTTGATACTATT
Hairpin Sequence:
5'-CCGG-CTGACGGAAGTTGATACTATT-CTCGAG-AATAGTATCAACTTCCGTCAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGACGGAAGTTGATACTATTCTCGAGAATAGTATCAACTTCCGTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGACGGAAGTTGATACTATTCTCGAGAATAGTATCAACTTCCGTCAG-3'

Other clones with same target sequence:

(none)