Construct: shRNA TRCN0000414709

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007080.2-286s21c1
DNA Barcode:
AGAATATGTAAATGGACAACT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LSM6 (11157)
Gene Description:
LSM6 homolog, U6 small nuclear RNA and mRNA degradation associated
Transcript:
RefSeq NM_007080.2 (NON-CURRENT)
Match location:
Position 286 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000414709)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAATATGTAAATGGACAACT
Hairpin Sequence:
5'-CCGG-AGAATATGTAAATGGACAACT-CTCGAG-AGTTGTCCATTTACATATTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAATATGTAAATGGACAACTCTCGAGAGTTGTCCATTTACATATTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAATATGTAAATGGACAACTCTCGAGAGTTGTCCATTTACATATTCT-3'

Other clones with same target sequence:

(none)