Construct: shRNA TRCN0000414732

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008879.3-2009s21c1
DNA Barcode:
TCCCGGTTCTGGATCTCATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lcp1 (18826)
Gene Description:
lymphocyte cytosolic protein 1
Transcript:
RefSeq NM_008879.3 (NON-CURRENT)
Match location:
Position 2009 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000414732)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCCGGTTCTGGATCTCATTG
Hairpin Sequence:
5'-CCGG-TCCCGGTTCTGGATCTCATTG-CTCGAG-CAATGAGATCCAGAACCGGGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCCGGTTCTGGATCTCATTGCTCGAGCAATGAGATCCAGAACCGGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCCGGTTCTGGATCTCATTGCTCGAGCAATGAGATCCAGAACCGGGA-3'

Other clones with same target sequence:

(none)