Construct: shRNA TRCN0000414742

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016206.2-977s21c1
DNA Barcode:
GAGTCCTGGCCTTATCCTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VGLL3 (389136)
Gene Description:
vestigial like family member 3
Transcript:
RefSeq NM_016206.2 (NON-CURRENT)
Match location:
Position 977 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000414742)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTCCTGGCCTTATCCTTTG
Hairpin Sequence:
5'-CCGG-GAGTCCTGGCCTTATCCTTTG-CTCGAG-CAAAGGATAAGGCCAGGACTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTCCTGGCCTTATCCTTTGCTCGAGCAAAGGATAAGGCCAGGACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTCCTGGCCTTATCCTTTGCTCGAGCAAAGGATAAGGCCAGGACTC-3'

Other clones with same target sequence:

(none)