Construct: shRNA TRCN0000414833

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004607.2-505s21c1
DNA Barcode:
GCTATGTGTTCAAGTAGTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TBCA (6902)
Gene Description:
tubulin folding cofactor A
Transcript:
RefSeq NM_004607.2 (CURRENT)
Match location:
Position 505 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000414833)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATGTGTTCAAGTAGTATG
Hairpin Sequence:
5'-CCGG-GCTATGTGTTCAAGTAGTATG-CTCGAG-CATACTACTTGAACACATAGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATGTGTTCAAGTAGTATGCTCGAGCATACTACTTGAACACATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATGTGTTCAAGTAGTATGCTCGAGCATACTACTTGAACACATAGC-3'

Other clones with same target sequence:

(none)