Construct: shRNA TRCN0000414869

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021629.3-594s21c1
DNA Barcode:
ATGCTCCCTCTGGTAATTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GNB4 (59345)
Gene Description:
G protein subunit beta 4
Transcript:
RefSeq NM_021629.3 (NON-CURRENT)
Match location:
Position 594 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000414869)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGCTCCCTCTGGTAATTATG
Hairpin Sequence:
5'-CCGC-ATGCTCCCTCTGGTAATTATG-CTCGAG-CATAATTACCAGAGGGAGCAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGCTCCCTCTGGTAATTATGCTCGAGCATAATTACCAGAGGGAGCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGCTCCCTCTGGTAATTATGCTCGAGCATAATTACCAGAGGGAGCAT-3'

Other clones with same target sequence:

(none)