Construct: shRNA TRCN0000414947

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005477.2-4666s21c1
DNA Barcode:
TTAACTGTGATTAGGAGATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HCN4 (10021)
Gene Description:
hyperpolarization activated cyclic nucleotide gated potassium channel 4
Transcript:
RefSeq NM_005477.2 (NON-CURRENT)
Match location:
Position 4666 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000414947)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTAACTGTGATTAGGAGATAT
Hairpin Sequence:
5'-CCGG-TTAACTGTGATTAGGAGATAT-CTCGAG-ATATCTCCTAATCACAGTTAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTAACTGTGATTAGGAGATATCTCGAGATATCTCCTAATCACAGTTAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTAACTGTGATTAGGAGATATCTCGAGATATCTCCTAATCACAGTTAA-3'

Other clones with same target sequence:

(none)