Construct: shRNA TRCN0000414982
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_015646.4-616s21c1
- DNA Barcode:
- CAATGGAACAACTGTGCATTC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RAP1B (5908)
- Gene Description:
- RAP1B, member of RAS oncogene family
- Transcript:
- RefSeq NM_015646.4 (NON-CURRENT)
- Match location:
- Position 616 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000414982)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CAATGGAACAACTGTGCATTC
- Hairpin Sequence:
-
5'-CCGG-CAATGGAACAACTGTGCATTC-CTCGAG-GAATGCACAGTTGTTCCATTG-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCAATGGAACAACTGTGCATTCCTCGAGGAATGCACAGTTGTTCCATTGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACAATGGAACAACTGTGCATTCCTCGAGGAATGCACAGTTGTTCCATTG-3'
Other clones with same target sequence:
(none)
