Construct: shRNA TRCN0000415008

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022780.3-1608s21c1
DNA Barcode:
AGAAGAGAACGTTCCATATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RMND5A (64795)
Gene Description:
required for meiotic nuclear division 5 homolog A
Transcript:
RefSeq NM_022780.3 (NON-CURRENT)
Match location:
Position 1608 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415008)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAAGAGAACGTTCCATATAA
Hairpin Sequence:
5'-CCGG-AGAAGAGAACGTTCCATATAA-CTCGAG-TTATATGGAACGTTCTCTTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAAGAGAACGTTCCATATAACTCGAGTTATATGGAACGTTCTCTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAAGAGAACGTTCCATATAACTCGAGTTATATGGAACGTTCTCTTCT-3'

Other clones with same target sequence:

(none)