Construct: shRNA TRCN0000415028

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007155.5-696s21c1
DNA Barcode:
TGACTTTCTCTCTGCGTCTGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZP3 (7784)
Gene Description:
zona pellucida glycoprotein 3
Transcript:
RefSeq NM_007155.5 (NON-CURRENT)
Match location:
Position 696 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415028)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGACTTTCTCTCTGCGTCTGA
Hairpin Sequence:
5'-CCGG-TGACTTTCTCTCTGCGTCTGA-CTCGAG-TCAGACGCAGAGAGAAAGTCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGACTTTCTCTCTGCGTCTGACTCGAGTCAGACGCAGAGAGAAAGTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGACTTTCTCTCTGCGTCTGACTCGAGTCAGACGCAGAGAGAAAGTCA-3'

Other clones with same target sequence:

(none)