Construct: shRNA TRCN0000415052

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175306.4-1519s21c1
DNA Barcode:
TCACGATTGAGATGTTGAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Phactr4 (100169)
Gene Description:
phosphatase and actin regulator 4
Transcript:
RefSeq NM_175306.4 (CURRENT)
Match location:
Position 1519 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415052)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCACGATTGAGATGTTGAAAG
Hairpin Sequence:
5'-CCGG-TCACGATTGAGATGTTGAAAG-CTCGAG-CTTTCAACATCTCAATCGTGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCACGATTGAGATGTTGAAAGCTCGAGCTTTCAACATCTCAATCGTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCACGATTGAGATGTTGAAAGCTCGAGCTTTCAACATCTCAATCGTGA-3'

Other clones with same target sequence:

(none)