Construct: shRNA TRCN0000415063

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172920.4-893s21c1
DNA Barcode:
ATGTTGCTTGTGACCCATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dpy19l1 (244745)
Gene Description:
dpy-19-like 1 (C. elegans)
Transcript:
RefSeq NM_172920.4 (CURRENT)
Match location:
Position 893 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415063)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGTTGCTTGTGACCCATATT
Hairpin Sequence:
5'-CCGG-ATGTTGCTTGTGACCCATATT-CTCGAG-AATATGGGTCACAAGCAACAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGTTGCTTGTGACCCATATTCTCGAGAATATGGGTCACAAGCAACATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGTTGCTTGTGACCCATATTCTCGAGAATATGGGTCACAAGCAACAT-3'

Other clones with same target sequence:

(none)