Construct: shRNA TRCN0000415072

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003736.2-817s21c1
DNA Barcode:
GTCAATGCCGAGATTACTTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCDHGB4 (8641)
Gene Description:
protocadherin gamma subfamily B, 4
Transcript:
RefSeq NM_003736.2 (NON-CURRENT)
Match location:
Position 817 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415072)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCAATGCCGAGATTACTTTC
Hairpin Sequence:
5'-CCGG-GTCAATGCCGAGATTACTTTC-CTCGAG-GAAAGTAATCTCGGCATTGAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCAATGCCGAGATTACTTTCCTCGAGGAAAGTAATCTCGGCATTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCAATGCCGAGATTACTTTCCTCGAGGAAAGTAATCTCGGCATTGAC-3'

Other clones with same target sequence:

(none)