Construct: shRNA TRCN0000415083

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144557.5-2964s21c1
DNA Barcode:
GGGAATTTGAGACCGAATATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Myrip (245049)
Gene Description:
myosin VIIA and Rab interacting protein
Transcript:
RefSeq NM_144557.5 (CURRENT)
Match location:
Position 2964 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415083)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGAATTTGAGACCGAATATC
Hairpin Sequence:
5'-CCGG-GGGAATTTGAGACCGAATATC-CTCGAG-GATATTCGGTCTCAAATTCCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGAATTTGAGACCGAATATCCTCGAGGATATTCGGTCTCAAATTCCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGAATTTGAGACCGAATATCCTCGAGGATATTCGGTCTCAAATTCCC-3'

Other clones with same target sequence:

(none)