Construct: shRNA TRCN0000415145

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144643.2-1340s21c1
DNA Barcode:
GCAGTTACAGTCTAGTATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCLT1 (132320)
Gene Description:
sodium channel and clathrin linker 1
Transcript:
RefSeq NM_144643.2 (NON-CURRENT)
Match location:
Position 1340 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415145)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTTACAGTCTAGTATAAA
Hairpin Sequence:
5'-CCGG-GCAGTTACAGTCTAGTATAAA-CTCGAG-TTTATACTAGACTGTAACTGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTTACAGTCTAGTATAAACTCGAGTTTATACTAGACTGTAACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTTACAGTCTAGTATAAACTCGAGTTTATACTAGACTGTAACTGC-3'

Other clones with same target sequence:

(none)