Construct: shRNA TRCN0000415175

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001054.3-733s21c1
DNA Barcode:
ACTGTGGACCTCATGGTTGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SULT1A2 (6799)
Gene Description:
sulfotransferase family 1A member 2
Transcript:
RefSeq NM_001054.3 (NON-CURRENT)
Match location:
Position 733 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415175)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTGTGGACCTCATGGTTGAG
Hairpin Sequence:
5'-CCGG-ACTGTGGACCTCATGGTTGAG-CTCGAG-CTCAACCATGAGGTCCACAGT-TTTTTTTGAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTGTGGACCTCATGGTTGAGCTCGAGCTCAACCATGAGGTCCACAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTGTGGACCTCATGGTTGAGCTCGAGCTCAACCATGAGGTCCACAGT-3'

Other clones with same target sequence:

(none)