Construct: shRNA TRCN0000415199

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006348.3-1045s21c1
DNA Barcode:
GATTTGCTTCGGAGGATTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COG5 (10466)
Gene Description:
component of oligomeric golgi complex 5
Transcript:
RefSeq NM_006348.3 (NON-CURRENT)
Match location:
Position 1045 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415199)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTTGCTTCGGAGGATTATT
Hairpin Sequence:
5'-CCGG-GATTTGCTTCGGAGGATTATT-CTCGAG-AATAATCCTCCGAAGCAAATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTTGCTTCGGAGGATTATTCTCGAGAATAATCCTCCGAAGCAAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTTGCTTCGGAGGATTATTCTCGAGAATAATCCTCCGAAGCAAATC-3'

Other clones with same target sequence:

(none)