Construct: shRNA TRCN0000415267

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032256.1-1644s21c1
DNA Barcode:
GCAACCTTGAGTGTAACTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEM117 (84216)
Gene Description:
transmembrane protein 117
Transcript:
RefSeq NM_032256.1 (NON-CURRENT)
Match location:
Position 1644 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415267)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACCTTGAGTGTAACTTTA
Hairpin Sequence:
5'-CCGG-GCAACCTTGAGTGTAACTTTA-CTCGAG-TAAAGTTACACTCAAGGTTGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACCTTGAGTGTAACTTTACTCGAGTAAAGTTACACTCAAGGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACCTTGAGTGTAACTTTACTCGAGTAAAGTTACACTCAAGGTTGC-3'

Other clones with same target sequence:

(none)