Construct: shRNA TRCN0000415334

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014513.2-234s21c1
DNA Barcode:
ACCACACTTTGCGCCTCATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIR2DS5 (3810)
Gene Description:
killer cell immunoglobulin like receptor, two Ig domains and short cytoplasmic tail 5
Transcript:
RefSeq NM_014513.2 (CURRENT)
Match location:
Position 234 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415334)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCACACTTTGCGCCTCATTG
Hairpin Sequence:
5'-CCGG-ACCACACTTTGCGCCTCATTG-CTCGAG-CAATGAGGCGCAAAGTGTGGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCACACTTTGCGCCTCATTGCTCGAGCAATGAGGCGCAAAGTGTGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCACACTTTGCGCCTCATTGCTCGAGCAATGAGGCGCAAAGTGTGGT-3'

Other clones with same target sequence:

(none)