Construct: shRNA TRCN0000415351

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015879.2-669s21c1
DNA Barcode:
TCTTCAGCATGTCGATGTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ST8SIA3 (51046)
Gene Description:
ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3
Transcript:
RefSeq NM_015879.2 (NON-CURRENT)
Match location:
Position 669 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415351)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTTCAGCATGTCGATGTAAT
Hairpin Sequence:
5'-CCGG-TCTTCAGCATGTCGATGTAAT-CTCGAG-ATTACATCGACATGCTGAAGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTTCAGCATGTCGATGTAATCTCGAGATTACATCGACATGCTGAAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTTCAGCATGTCGATGTAATCTCGAGATTACATCGACATGCTGAAGA-3'

Other clones with same target sequence:

(none)