Construct: shRNA TRCN0000415353

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207405.1-1007s21c1
DNA Barcode:
AGCAATCAGAGCCCTCATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C4orf50 (389197)
Gene Description:
chromosome 4 open reading frame 50
Transcript:
RefSeq NM_207405.1 (NON-CURRENT)
Match location:
Position 1007 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415353)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAATCAGAGCCCTCATTTA
Hairpin Sequence:
5'-CCGG-AGCAATCAGAGCCCTCATTTA-CTCGAG-TAAATGAGGGCTCTGATTGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAATCAGAGCCCTCATTTACTCGAGTAAATGAGGGCTCTGATTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAATCAGAGCCCTCATTTACTCGAGTAAATGAGGGCTCTGATTGCT-3'

Other clones with same target sequence:

(none)