Construct: shRNA TRCN0000415355

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002338.3-1202s21c1
DNA Barcode:
GACGACAAGCTTCACTCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LSAMP (4045)
Gene Description:
limbic system associated membrane protein
Transcript:
RefSeq NM_002338.3 (NON-CURRENT)
Match location:
Position 1202 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415355)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACGACAAGCTTCACTCAAAT
Hairpin Sequence:
5'-CCGG-GACGACAAGCTTCACTCAAAT-CTCGAG-ATTTGAGTGAAGCTTGTCGTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACGACAAGCTTCACTCAAATCTCGAGATTTGAGTGAAGCTTGTCGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACGACAAGCTTCACTCAAATCTCGAGATTTGAGTGAAGCTTGTCGTC-3'

Other clones with same target sequence:

(none)