Construct: shRNA TRCN0000415520

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000493.3-2571s21c1
DNA Barcode:
CATCCAGGAGGTATCATATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COL10A1 (1300)
Gene Description:
collagen type X alpha 1 chain
Transcript:
RefSeq NM_000493.3 (NON-CURRENT)
Match location:
Position 2571 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415520)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCCAGGAGGTATCATATAA
Hairpin Sequence:
5'-CCGG-CATCCAGGAGGTATCATATAA-CTCGAG-TTATATGATACCTCCTGGATG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCCAGGAGGTATCATATAACTCGAGTTATATGATACCTCCTGGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCCAGGAGGTATCATATAACTCGAGTTATATGATACCTCCTGGATG-3'

Other clones with same target sequence:

(none)