Construct: shRNA TRCN0000415641

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033393.2-1159s21c1
DNA Barcode:
GTTCAGAAGACTGCTAGATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FHDC1 (85462)
Gene Description:
FH2 domain containing 1
Transcript:
RefSeq NM_033393.2 (CURRENT)
Match location:
Position 1159 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415641)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCAGAAGACTGCTAGATTA
Hairpin Sequence:
5'-CCGG-GTTCAGAAGACTGCTAGATTA-CTCGAG-TAATCTAGCAGTCTTCTGAAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCAGAAGACTGCTAGATTACTCGAGTAATCTAGCAGTCTTCTGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCAGAAGACTGCTAGATTACTCGAGTAATCTAGCAGTCTTCTGAAC-3'

Other clones with same target sequence:

(none)