Construct: shRNA TRCN0000415665

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014191.2-1477s21c1
DNA Barcode:
AGCAATGTTGGAGCAACTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCN8A (6334)
Gene Description:
sodium voltage-gated channel alpha subunit 8
Transcript:
RefSeq NM_014191.2 (NON-CURRENT)
Match location:
Position 1477 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415665)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAATGTTGGAGCAACTTAA
Hairpin Sequence:
5'-CCGG-AGCAATGTTGGAGCAACTTAA-CTCGAG-TTAAGTTGCTCCAACATTGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAATGTTGGAGCAACTTAACTCGAGTTAAGTTGCTCCAACATTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAATGTTGGAGCAACTTAACTCGAGTTAAGTTGCTCCAACATTGCT-3'

Other clones with same target sequence:

(none)