Construct: shRNA TRCN0000415680

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001472.2-225s21c1
DNA Barcode:
CAACGTCAGGATCCTGCAGCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GAGE2C (2574)
Gene Description:
G antigen 2C
Transcript:
RefSeq NM_001472.2 (CURRENT)
Match location:
Position 225 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415680)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACGTCAGGATCCTGCAGCT
Hairpin Sequence:
5'-CCGG-CAACGTCAGGATCCTGCAGCT-CTCGAG-AGCTGCAGGATCCTGACGTTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACGTCAGGATCCTGCAGCTCTCGAGAGCTGCAGGATCCTGACGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACGTCAGGATCCTGCAGCTCTCGAGAGCTGCAGGATCCTGACGTTG-3'

Other clones with same target sequence:

(none)