Construct: shRNA TRCN0000415686

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020240.2-960s21c1
DNA Barcode:
TGTCAGACGAATCCTGCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDC42SE2 (56990)
Gene Description:
CDC42 small effector 2
Transcript:
RefSeq NM_020240.2 (CURRENT)
Match location:
Position 960 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415686)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTCAGACGAATCCTGCATTT
Hairpin Sequence:
5'-CCGG-TGTCAGACGAATCCTGCATTT-CTCGAG-AAATGCAGGATTCGTCTGACA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTCAGACGAATCCTGCATTTCTCGAGAAATGCAGGATTCGTCTGACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTCAGACGAATCCTGCATTTCTCGAGAAATGCAGGATTCGTCTGACA-3'

Other clones with same target sequence:

(none)