Construct: shRNA TRCN0000415698

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024953.2-982s21c1
DNA Barcode:
GAGGACCACATCTAGCTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NAA25 (80018)
Gene Description:
N(alpha)-acetyltransferase 25, NatB auxiliary subunit
Transcript:
RefSeq NM_024953.2 (NON-CURRENT)
Match location:
Position 982 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415698)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGACCACATCTAGCTAAAT
Hairpin Sequence:
5'-CCGG-GAGGACCACATCTAGCTAAAT-CTCGAG-ATTTAGCTAGATGTGGTCCTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGACCACATCTAGCTAAATCTCGAGATTTAGCTAGATGTGGTCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGACCACATCTAGCTAAATCTCGAGATTTAGCTAGATGTGGTCCTC-3'

Other clones with same target sequence:

(none)